Fig. 2: Genetic alterations in the tTA driver and lethal effector vectors in primary-site lethal revertant survivor lines.
From: Genetic breakdown of a Tet-off conditional lethality system for insect population control
a Line 18-f1 has a 27-bp deletion and 3 nucleotide substitutions (blue vertical lines and nucleotides) in the tTA gene and b line 45-f1 has a 26-bp deletion in the hidAla5 cell death gene (maroon dashes and triangles indicate deleted sequences). Lines 9-f1, 62-f1, and 65-m2 have identical deletions of the TREp-hidAla5 lethal effector and two 5’ HS4 insulator sequences, lines 44-m1 and 21-f1 exhibit deletions (blue dotted lines), and insert sequences (blue solid lines) from within the ECFP-linked SV40 extending downstream through the 3’ genomic insertion site sequence (blue arrows indicate unknown indel termination sites).
