Table 1 Tet-off lethality revertant adult survivors on Tet-free diet.

From: Genetic breakdown of a Tet-off conditional lethality system for insect population control

Matings

No. of P1 matingsa

Diet

F1 R/C survivors

Fertile F1 R/C survivors

F2 R/C survivor linesb

Primary-site revertants

Second-site revertants

Control

6

Tet

90,006c

—

—

—

—

Experimental

80

Tet-free

109

73

20

7

13

 Survival frequencyd

  

9.1 ± 0.45 × 10−5

6.1 ± 0.30 × 10−5

1.7 ± 0.083 × 10−5

5.8 ± 0.29 × 10−6

1.1 ± 0.054 × 10−5

 Modified survival frequencye

  

8.9 ± 0.44 × 10−5

5.9 ± 0.29 × 10−5

1.4 ± 0.071 × 10−5

3.3 ± 0.17 × 10−6

1.1 ± 0.054 × 10−5

  1. a400 w[m] females mated to 100 DH-1 double homozygous driver/effector (PUb-DsRed/3xP3-Cyan; R/C) males.
  2. bF2 R/C progeny from individual fertile F1 R/C backcross matings to w[m].
  3. cCalculated from 15,001 ± 389 SEM surviving F1 adults per control mating.
  4. dNo. of R/C marked F1 survivors/~1,200,000 ±SEM adults screened (estimation based on surviving F1 adults from each control mating on Tet-diet).
  5. eModified survival frequency data based on the deletion of the primary-site revertant lines 9-f1, 62-f1, and 65-m2 whose survival may have resulted from cis-recombination between the 5’ HS4 repetitive insulator sequences that are not required for lethality system function, which does not affect the second-site revertant frequency; frequencies presented as confidence intervals at the 95% confidence level.
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