Fig. 1: Recording of CA1 LFP in behaving mouse.
a A multielectrode array was dorsally implanted into the CA1 7 d before 2VO experimentation. Recordings of LFP from the dorsal hippocampal CA1 region in vivo were performed on free-moving mice. b–d, LFP PSD of CA1 during light flicker. e–g Quantification of LFP PSD of the three groups of mice with the same legend shown in g (n = 6 sessions/5 mice each group). h Example spectrograms for the four groups of mice recorded 10 s in CA1 during low-speed free walking. The excerpt of raw LFPs of theta, low gamma, and high gamma oscillations are shown in the lower panel of h, and the PSD intensity is color-coded according to the color scale shown on the right. i–k Quantification of the LFP powers of the four groups of mice in the theta, low gamma, and high gamma frequencies. The same group legend is shown in k (Sham: n = 5 sessions/3 mice; Sham+LED: n = 6 sessions/3 mice; 2VO: n = 7 sessions/3 mice; 2VO + LED; n = 8 sessions/3 mice). l,m PAC analysis of low gamma and high gamma, respectively, to the theta phase as indicated by the modulation index (n = 3 mice). n Example of moving window spectrogram of hippocampal CA1 LFP time-locked to the initiation of approach (<0 s) and explore (>0 s) a novel object and the PSD intensity is color-coded according to the color scale shown on the right. o–r The ratio of PSD between Exploration (PSDExp) and Approach (PSDAppr) of the four groups of mice at the indicated oscillation frequencies on the y-axis (n = 6 sessions/3 mice). Data represent the mean ± SEM. Error bars indicate SEM. *P < 0.05, ##P < 0.01, *** and ###P < 0.001; The test used in e–g 1ANOVA with LSD’s post hoc analysis; i–k 2ANOVA with LSD’s post hoc test; l, m 2ANOVA with Tukey's post hoc test; o–r RM-2ANOVA with Sidak’s post hoc test. Source data are provided as a Source Data file.
