Fig. 5: 40 Hz light stimulation enhanced CA3-CA1 presynaptic short-term plasticity.
a Hippocampal slices from the Sham, Sham+LED, 2VO, and 2VO + LED groups at 3 d after the 2VO surgery were subjected to PPR measurements. The inter-stimulus intervals (ISI) were from 20–200 ms. The ratios of the slope of the second response to that of the first at the 40 ms ISI for a were measured and plotted in b (Sham: n = 5 mice; Sham+LED: n = 5 mice; 2VO: n = 4 mice; 2VO + LED: n = 5 mice). c The protocol for determining the kinetics of FM1-43 release from presynaptic terminals. Presynaptic terminals were labeled by exposure to 5 µM FM1-43 (numbers indicate time in minutes and seconds; zero time represents the beginning of the unloading stimulation; Scale bar = 2 µm). The first image was taken before unloading stimulation. d Normalized fluorescence intensity decay of puncta to the initial FM1-43 loading intensity were plotted. Each point represents the average of a total of 10–15 boutons from different slices of mice. The rate of puncta unloading in brain slices (1/t1/2) was plotted and shown in e (Sham: n = 3 mice; Sham+LED: n = 3 mice; 2VO: n = 3 mice; 2VO + LED: n = 4 mice). Data represent the mean ± SEM. Error bars indicate SEM. ***, ###P < 0.001. The test used in b, e 2ANOVA with Tukey’ post hoc test. Source data are provided as a Source Data file.
