Fig. 4: Restricted myeloid expression of Cdx2 causes disease distinct from Scl:Cdx2.

a Diagram of breeding strategy to product LysM:Cdx2 mice. LSL-Cdx2-mCherry mice are crossed with LysM-Cre knock-in mice at the endogenous LysM locus, leading to LysM-expressing GMP cells and their progeny to express Cdx2 and mCherry-reporter proteins. b Representative flow cytometry plots showing mCherry expression in PB subsets of LysM-Cre (blue shaded) and LysM:Cdx2 (red line) mice. c Representative flow cytometry plots showing mCherry expression in BM subsets of LysM-Cre (grey shaded) and LysM:Cdx2 (red line) mice. d Survival curve after 60 weeks of monitoring (LysM-Cre, n = 23; LysM:Cdx2, n = 16). e Proportion of hematological state of LysM:Cdx2 mice at sacrifice. f Box plot of quantification of neutrophil nuclei segments in PB smears. Top and bottom of whiskers represent maximum and minimum values respectively, centre line within box represents median value, upper and lower quartiles represent 75th and 25th percentile respectively. At least 15 neutrophils were counted per slide by independent researchers, n = 4 per group. g Wright-Giemsa stained PB smears showing representative neutrophils of LysM-Cre and LysM:Cdx2 mice. Scale bars on bottom left of each image indicate 10 µm. h PB leukocyte (WBC) and i hemoglobin levels of mice at sacrifice (LysM-Cre n = 12; LysM:Cdx2 n = 9). j Spleen size relative to body mass of mice at sacrifice (LysM-Cre n = 7; LysM:Cdx2 n = 9). N = biologically independent animals. Statistical analyses performed using two-tailed Mann–Whitney test. Data are plotted as mean values +/− SD. n.s.; not significant. **P < 0.01, ****P < 0.0001.