Fig. 5: Cdx2 confers a progenitor gene signature associated with differentiation. | Nature Communications

Fig. 5: Cdx2 confers a progenitor gene signature associated with differentiation.

From: Hematopoietic stem and progenitor cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia

Fig. 5

a Heatmap and unsupervised hierarchical clustering of top 50 differentially expressed genes from comparing Scl:Cdx2 and Scl-Cre on normalized read counts from RNA-Seq of BM LKS + cells of Scl-Cre, Scl:Cdx2, Scl/Flt3ITD/+, and Scl:Cdx2/Flt3ITD/+ mice. Color scale shows Z-score after row normalization. b Principal component analysis of the total RNA-Seq derived transcriptome. c–h Gene Set Enrichment Analysis (GSEA) on normalized read counts of transcripts from RNA-Seq of Scl-Cre (n = 2) compared with Scl:Cdx2 (n = 3). i Cell cycle analysis of BM LKS+ cells (Scl-cre n = 11; Scl:Cdx2 n = 7). j Apoptosis analysis of Annexin V-positive, Sytox-negative cells in BM LKS + by flow cytometry (Scl-cre n = 12; Scl:Cdx2 n = 8). N = biologically independent animals. Statistical analyses of graphs performed using two-tailed Mann–Whitney test. Data are plotted as mean values +/− SD. n.s.; not significant. **P < 0.01, ***P < 0.001, ****P < 0.0001.

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