Table 1 Details of antibodies used.
Serial No. | Antibody | Cat. no. | Company | Application (dilution) | |||
---|---|---|---|---|---|---|---|
IFA/FACS | Confocal | WB | IHC | ||||
1 | MAP1LC3B | NB100-2220 | NovusBiological | 1:200 | 1:1000 | ||
2 | GAPDH | sc-48167 | Santa Cruz Biotechnology | 1:5000 | |||
3 | COX2 | ab62331 | Abcam | 1:1000 | |||
4 | Rab5 | sc-309 | Santa Cruz Biotechnology | 1:200 | |||
5 | Rab7 | sc-6563 | Santa Cruz Biotechnology | 1:200 | |||
6 | ABCC1 | sc-18835 | Santa Cruz Biotechnology | 1:100 | 1:100 | ||
7 | LAMP1 | sc-20011 | Santa Cruz Biotechnology | 1:200 | |||
8 | ABCG2 | sc-58222 | Santa Cruz Biotechnology | 1:100 | 1:100 | ||
9 | Cathepsin D | ab19555 | Abcam | 1:200 | |||
10 | CD73-FITC (clone AD2) | 561254 | BD Bioscience | 1:100 | |||
11 | CD11b-PE | 557321 | BD Bioscience | 1:100 | |||
12 | CD271 | ab8874 | Abcam | 1:100 | |||
13 | CD44 | MS668P | Thermo Scientific | 1:100 | |||
14 | CD90 | ab181469 | Abcam | 1:100 | |||
15 | CD105 | ab114052 | Abcam | 1:100 | |||
16 | CD45-APC (clone 30-F11) | 559864 | BD Bioscience | 1:100 | |||
17 | CD73-BV450 (clone TY/23) | 561544 | BD Bioscience | 1:50 | |||
18 | CD11b-APC-Cy7 (clone M1/70) | 557657 | BD Bioscience | 1:100 | |||
19 | Ly6G−PE (clone 1A8) | 551461 | BD Bioscience | 1:100 | |||
20 | Sca-1-PE-CF594 (clone D7) | 562730 | BD Bioscience | 1:100 | |||
21 | CD90-BB515 (clone OX7) | 564607 | BD Bioscience | 1:100 | |||
22 | CD44-BV605 (Clone IM7) | 563058 | BD Bioscience | 1:100 | |||
23 | CD105-PE (Clone MJ7/18) | 562759 | BD Bioscience | 1:100 | |||
24 | Ly6c-BV605 (Clone AL-21) | 563011 | BD Bioscience | 1:100 | |||
25 | I-A/I-E-BB700 (clone 2G9) | 746086 | BD Bioscience | 1:100 | |||
26 | CD11c-PE-Cy7 (clone HL3) | 558079 | BD Bioscience | 1:100 | |||
27 | Rabbit anti-Ag85B | ab43019 | Abcam | 1:2000 | 1:2000 | ||
28 | Mouse anti-CD73 (1D7) | ab91086 | Abcam | 1:1600 | 1:500 | ||
29 | Mouse anti-105 (SN6) | ab11414 | Abcam | 1:500 | |||
30 | Rabbit IgG1 polyclonal Isotype control | ab37415 | Abcam | 1:2000 | 1:2000 | ||
31 | Mouse IgG1 kappa monoclonal Isotype control | ab81032 | Abcam | 1:1600;1:500 | 1:500 | ||
32 | Goat anti-rabbit Alexa fluor 405,488, 568, 647 | A31556 A11034 A11011 A21245 | Thermo Scientific | 1:200 | 1:400 | 1:200 | |
33 | Goat anti-mouse Alexa fluor 405,488, 568, 647 | A31553 A28175 A11031 A21235 | Thermo Scientific | 1:200 | 1:400 | 1:200 |