Fig. 3: Comparison of type I–F PaeCascade and other VPR-based systems.
From: Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells
a Schematic illustrating of the gRNAs and crRNAs of dCas9, dAsCas12a, EcoCascade, and PaeCascade targeting different regions upstream the transcriptional start site (TSS) of four genes. Arrow directions indicated the 5′ to 3′ sequence of the spacer. Colored lines (purple, green, yellow, and blue) represented the PAM motif. b Quantitative PCR analysis of HBB transcription levels in HEK293T cells transfected with dCas9-VPR, dAsCas12a-VPR, EcoCascade-VPR, or PaeCascade VPR (Csy3-VPR). c Quantitative PCR analysis of HBG transcription levels in HEK293T cells transfected with dCas9-VPR, dAsCas12a-VPR, EcoCascade-VPR, or PaeCascade VPR (Csy3-VPR). d Quantitative PCR analysis of SOX2 transcription levels in HEK293T cells transfected with dCas9-VPR, dAsCas12a-VPR, EcoCascade-VPR, or PaeCascade VPR (Csy3-VPR). e Quantitative PCR analysis of IL1B transcription levels in HEK293T cells transfected with dCas9-VPR, dAsCas12a-VPR, EcoCascade-VPR, or PaeCascade VPR (Csy3-VPR). Ctrl: non-targeting crRNA control. Data represented three biological repeats and displayed as mean ± S.E.M. Statistical significance was calculated using one-way ANOVA (*P < 0.05; ***P < 0.001). Source data are provided as a Source Data file.
