Fig. 2: Chronic circadian disruption increases cancer-cell dissemination and metastasis. | Nature Communications

Fig. 2: Chronic circadian disruption increases cancer-cell dissemination and metastasis.

From: Chronic circadian disruption modulates breast cancer stemness and immune microenvironment to drive metastasis in mice

Fig. 2

a Disseminated tumour cells detected in LD and JL mice at 16 weeks of age by flow cytometry (left, LD n = 14 and JL n = 11) and real-time PCR (right, LD n = 14 and JL n = 12) of bone marrow mononucleated cells (BM-MNC). Representative flow cytometry plots showing intracellular PyMT staining of CD45−CD31−CD140a−Ter119− live BM-MNC cells. Data are presented as scatter dot plots with lines indicating the median with interquartile range (error bars). P-values are calculated from an unpaired two-sided t-test. b Circulating tumour cells in peripheral blood mononuclear cells (PBMCs) detected by flow cytometry (left; LD: n = 8 and JL: n = 6) and real-time qPCR (right; LD: n = 12 and JL: n = 9) in mice at 16 weeks of age. Representative flow cytometry plots showing intracellular PyMT staining of CD45− live PBMCs (SSC: side scatter). Data are presented as scatter dot plots with lines indicating the median with interquartile range (error bars). P-values are calculated from an unpaired two-sided t-test. c Prevalence of lung metastasis in LD (n = 25) and JL (n = 21) cohorts at 16 weeks of age. Data represent the percentage of mice with metastasis in both conditions, P-value obtained from a binomial two-sided test. d Number of metastatic foci per lung in LD (n = 25) and JL (n = 21) mice at 16 weeks of age. Data represent the percentage of mice with >3 or <3 metastatic foci in both conditions. P-value obtained from a binomial two-sided test. Indicated (n) represents the number of independent experiments as biological replicates.

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