Fig. 3: Functional comparison of different ORK designs.
From: Phosphoregulated orthogonal signal transduction in mammalian cells
a ORKs consisting of different N-terminal truncation variants of DcuS fused N-terminally or C-terminally to the nanobody acVHH, which dimerizes in the presence of caffeine. b Reporter gene expression in response to caffeine for POST with N-terminal or C-terminal acVHH fusion ORKs, co-expressed with the OGR (DcuR-VP16). The bar chart shows the mean ± s.d. of n = 3 biologically independent samples overlaid with a scatter dot plot of the original data points, measured 24 h after induction and the results are representative of three independent experiments. Source data are provided as a Source Data file.
