Fig. 4: POST as a dimerization relay system.
From: Phosphoregulated orthogonal signal transduction in mammalian cells
a Schematic of the proposed basic building block of protein circuits using a phosphoregulated relay system of protein dimerization. This system consists of a dimerization-dependent receptor and a dimerization-dependent effector. b Schematic of POST as a dimerization relay system by a two-hybrid OGR design. The two-hybrid OGR serves as an example of a phosphoregulated effector protein that performs its function upon dimerization. It consists of DcuR fused to TetR, and DcuR fused to the DNA transactivation domain VPR. Phosphoregulation by ORKs controls the dimerization of TetR-DcuR and DcuR-VPR and only dimerized TetR-DcuR/DcuR-VPR will induce reporter gene expression from a TetR operator. c Reporter gene expression from a TetR operator in response to caffeine in the two-hybrid OGR design. The bar chart shows the mean ± s.d. of n = 3 biologically independent samples overlaid with a scatter dot plot of the original data points, measured at 24 h after induction, and the results are representative of three independent experiments. Source data are provided as a Source Data file.
