Fig. 5: Dose–response curves.
From: Phosphoregulated orthogonal signal transduction in mammalian cells
a The dose–response curves of caffeine-induced reporter gene expression for POST with ORKs containing DcuS324–543 or DcuS330–543 fused to an N-terminal acVHH dimerization domain. b The dose–response curves for ORKs with a C-terminal acVHH dimerization domain. For better comparability with the performance of N-terminal acVHH fusion ORKs, the Y-axis has the same scale as panel a. c Dose–response of the two-hybrid OGR design and an acVHH-DcuS324–543 ORK. Plots show the mean ± s.d. of n = 3 biologically independent samples measured 24 h after induction, and the results are representative of three independent experiments. Only error bars larger than the symbol size are displayed. Source data are provided as a Source Data file.
