Fig. 1: MeCP2 is physically associated with nucleosome complexes.
From: MeCP2 regulates gene expression through recognition of H3K27me3
a MeCP2 antibody immunoprecipitated proteins from mouse OE nuclear extract in SDS gel visualized with Coomassie blue. Nuclear extract is loaded as input; rabbit IgG as a negative control. Star is around 25–30 kDa. b Benzonase treated nuclear extracts were immunoprecipitated either with anti-MeCP2 antibody or anti-histone H1 antibody. Samples of input, IgG and immunoprecipitates were analyzed with western blotting for histone H1 or MeCP2. c Benzonase treated nuclear extracts were immunoprecipitated with a MeCP2 antibody specific to its C-terminus. Input, IgG control and immunoprecipitates were analyzed with Western blotting for the presence of histone H3 and H4. d Z-transformed aggregate plot of the average tag density of MeCP2 binding sites (hotspot, green) at chr19 occupied by: Nucleosome occupancies (gray) and histone H1 (purple). e Z-transformed aggregate plot of the average tag density of MNase-seq (hotspot, gray) at chr19 occupied by: MeCP2 (green) and histone H1 (purple). f Genome-wide inter-correlation among Input, histone H1 ChIP-seq, MeCP2 ChIP-seq, and MNase-seq (binning size = 150 bp). Number in the square indicates the Pearson correlation coefficient. Source data are provided as a Source data file (b and c). The shaded areas are up to ±S.D. from the average profile (d and e).
