Fig. 5: MeCP2 binds to H3K27me3 enriched loci independent of DNA methylation.
From: MeCP2 regulates gene expression through recognition of H3K27me3
a MeCP2 enrichment at differential methylated regions (DMRs) between HCT116 and DKO1 cells. N = 2 (per genotype, HCT116 vs DKO1) from biologically independent cells. n = 91,106, p < 0.0001 (two-tailed Wilcoxon signed-rank test, ***p < 0.0001). Box-and-whisker plots show median, 10–90 percentile, and min and max values. p = 0.00 were reported as p < 0.0001. b Genome-wide inter-correlation among Input, H3K27me3 ChIP-seq, MeCP2 ChIP-seq in both HCT116 and DKO1 (binning size = 200 bp). Global Pearson correlation analyses between input, H3K27me3 and MeCP2 across HCT116 and DKO1. c Venn diagram showing the number of common and unique H3K27me3 peaks between HCT116 and DKO1. d Heatmap illustrating randomly selected 5000 loci with increased H3K27me3 in DKO1. Peaks are sorted according to signal intensity. Averaged H3K27me3 signals are given for HCT116 (purple) and DKO1 (green). e Heatmap showing changes in MeCP2 occupancy within corresponding H3K27me3 increased loci of DKO1 shown in d. Peaks are sorted according to signal intensity. Averaged MeCP2 signals are given for HCT116 (purple) and DKO1 (green). f Representative tracks of MeCP2 binding, DNA methylation and H3K27me3 enrichment in HCT116 and DKO1 cells at chr11: 129,230,000–129,250,000. UCSC genome-browser view (hg19) of the CpG density (per 150 bp) is shown on top. DMR is indicated in green bar. MeCP2 and H3K27me3 enrichment are input normalized. The shaded areas are up to ±S.D. from the average profile (d and e).
