Fig. 6: Coexistence of MeCP2, H3K27me3, and H3K9ac in transcription regulatory regions.
From: MeCP2 regulates gene expression through recognition of H3K27me3
a Genome-wide distribution of MeCP2 binding loci, determined by PING. Mouse OE is the tissue source for all subsequent analyses in this figure. b MeCP2 binding frequency determined by Z-score in each annotated gene structural regions. c UCSC genome-browser view (mm9) of MeCP2, H3K27me3, H3K9ac ChIP-seq, and DNA methylation levels at a region on chr17: 45,675,956–45,700,322. The transparent blue bars indicate loci where MeCP2 and H3K27me3 coexist. The transparent orange bars point to the co-enrichment of MeCP2, H3K27me3, and H3K9ac. d Percentage of overlap between MeCP2 binding and H3K27me3 (blue) or H3K27me3 with H3K9ac (orange) within each annotated regions. e Methylation levels of CpG, CAG and CAH in 1 kb up- and 4 kb downstream of the TSS. f Z-score transformed average enrichment of MeCP2, H3K27me3, and H3K9ac, calculated 1 kb up- and 4 kb downstream of the TSS. g Methylation levels of CpG, CAG and CAH in 3 kb up- and 3 kb downstream of the enhancer loci (defined in Supplementary Fig. 9G). h Z-score transformed average enrichment of MeCP2, H3K27me3 and H3K9ac, calculated −3 kb to +3 kb around the midpoint of each enhancer (defined in Supplementary Fig. 9G). The shaded areas are up to ±S.D. from the average profile (e-h).
