Fig. 1: AS-MS enables discovery from randomized, high-diversity chemical libraries.
From: Ultra-large chemical libraries for the discovery of high-affinity peptide binders
a Among existing techniques for peptide binder discovery, increased chemical control over library synthesis and screening typically comes at the cost of limited library diversity. Affinity selection-mass spectrometry (AS-MS; this work), which relies on chemically accessed libraries and direct identification of active members, can investigate library diversities on the order of 108–109 while maintaining a high degree of chemical control over the synthesis and selection process. b A typical AS-MS workflow, which uses magnetic beads as a partitioning reagent to discriminate bound from unbound library members, and nano-liquid chromatography-tandem mass spectrometry to identify sequences of active peptides.
