Table 1 Cryo-EM data collection, refinement and validation statistics.
FliDcj (EMDB-10210) (PDB: 6SIH) | Filament (EMDB-10244) | |
|---|---|---|
Data collection and processing | ||
Microscope | Titan Krios | Tecnai Arctica |
Magnification | 36232 | 53000 |
Voltage (kV) | 300 | 200 |
Camera | K2 Summit | Falcon III |
Pixel size (Å) | 1.38 | 2.03 |
Symmetry Imposed | D5 (full map), C5 (head map) | Helical (Rise: 7.25 Å, Twist: 65.4°) |
Defocus range (µm) | −1.0 to −2.6 | −0.8 to −2.0 |
Total dose (eÅ−2) | 41 | 45 |
Number of micrographs | 1223 | 100 |
Total particles used | 55967 | 71828 |
Map Resolution (Å) | 4.7 (full map) 5.0 (head domain) | 8.6 (symmetrical) 27.2 (asymmetrical) |
Refinement | ||
Map sharpening B factor (Å2) | −285 | |
Model composition | ||
Non-hydrogen atoms | 37320 | |
Protein Residues | 4880 | |
B factors (Å−1) | ||
Protein | 148 | |
R.m.s. deviations | ||
Bond lengths (Å) | 0.005 | |
Bond angles (°) | 0.543 | |
Validation | ||
MolProbity score | 1.85 | |
Clashscore | 8.36 | |
Poor rotamers (%) | 0.00 | |
Ramachandran plot | ||
Favored (%) | 94.13 | |
Allowed (%) | 5.87 | |
Disallowed (%) | 0.00 | |
