Fig. 3: Rescue of tau phenotypes in 8-week differentiated A152T (FTD19-L5-RC6) neurons upon 24 h treatment.

a Summary of the assay for protein fractionation based on Triton-X (T) and SDS (S) detergents differential solubility. b–d Western blot analysis of tau after mTORi compound treatment and detergent fractionation. b Samples were run on the same blot, image cropped (dotted line) only for the purpose of this figure. Brackets indicate protein bands for densitometry analysis. Graph bars represent mean densitometry ± SEM, and black diamond-dots indicate individual data points for soluble (c) and insoluble (d) total tau (TAU5) and P-tau^S396 levels relative to vehicle samples. n = 4 biological replicates. Statistical significance relative to vehicle was calculated using two-tailed unpaired t-test and P values are indicated in each graph. e–l Rescue of neuronal vulnerability to 30 μM Aβ(1-42) (red) and 400 μM NMDA (blue), by pre-treatment with rapamycin (e, i), OSI-027 (f, j), AZD2014 (g, k) or AZD8055 (h, l). Assay summary in Supplementary Fig. 4f. Bars represent mean % viability relative to vehicle-treated neurons ± SEM, and black diamond-dots represent individual data points for n = 3 biological replicates with 2 technical replicates per experiment. Statistical significance was calculated with a two-tailed unpaired t-test and P values are indicated (^nsP > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001). Source data are provided as a Source Data file.