Fig. 6: Treatment with GalNAc-siMCJ attenuates fatty liver disease.

a Mice received a single s.c. dose of GalNAc-si381 (10 mg/Kg), GalNAc-si393, or PBS. MCJ expression in the liver was analyzed 5 days later by Western blot analysis. b Mice (n = 4) received a single dose of GalNAc-si381 or GalNAc-si393 (10 mg/Kg), or PBS. After 5 days they were fasted for 36 h. Liver lipids were quantified by Oil Red O staining. c–e Mice were administered with a s.c. dose of GalNAc-si381 or GalNAc-si393 (n = 7), or PBS (n = 6) at the time they started on the MCD diet. A second dose was administered 2 weeks later. Mice were harvested after 1 additional week. c Representative images of liver lipid accumulation by Oil Red O staining. d Quantitation of liver lipids. e Quantitation of liver fibrosis by Picro Sirius Red staining. f and g Mice (n = 7) were fed choline-deficient high-fat diet (CD-HFD) for 4 months prior to the treatment with a s.c. dose of GalNAc si381 or PBS every 2 weeks for 2 more months on the diet (4 doses total). Tissues were harvested 2-weeks after the last dose. (f) Representative images of H&E stained-liver sections. (g) Quantification of liver lipids by Oil Red O staining. Percentage of total histological area is shown. h–k After 2 weeks on mMCD diet (pre-treatment), mice (n = 5) received s.c. administration of GalNAc-si393 or PBS every 2 weeks for 4 additional weeks (2 doses total). Tissues were harvested 2 weeks after the last dose. h Quantitation of liver lipid by Oil Red O staining of liver sections. i Quantitation of liver fibrosis by Picro Sirius Red staining (number of fibrotic areas per section). j Representative liver images of Picro Sirius Red staining. k Relative expression of smooth muscle actin (Acta 2) mRNA levels by real-time RT-PCR and β2-microglobulin as house-keeping gene. *denotes p < 0.05, as determined by one-way ANOVA or t test. Error bars show standard deviation (SD) in all panels. Source data are provided as a Source Data file.