Fig. 7: LincNMR depletion reduces dNTP levels while supplying dNTPs rescues the lincNMR phenotype.

a Quantification of dNTP levels uncovers that the depletion of lincNMR with 10 nM siPOOLs leads to strong downregulation of dATP, dCTP, dGTP, and dTTP in HLE cells (n = 3). b Quantification of dNTP levels uncovers that the depletion of lincNMR with 10 nM siPOOLs leads to strong downregulation of dATP, dCTP, dGTP, and dTTP in FLC-4 cells (n = 3). c Quantification of dNTP levels uncovers that the depletion of YBX1 with 10 nM siPOOLs leads to downregulation of dATP, dCTP, dGTP, and dTTP in FLC-4 cells (n = 3). d Quantification of dNTP levels uncovers that the depletion of YBX1 with 10 nM siPOOLs leads to downregulation of dATP, dCTP, dGTP, and dTTP in FLC-4 cells (n = 3). e Schematic outline of dNTP bathing & rescue assay. f Supplying dNTPs rescues dose-dependently the proliferation decrease caused by lincNMR silencing determined by a BrdU incorporation assay. HLE cells were reverse transfected with 10 nM of the respective siPOOLs and bathed in increasing concentrations (0–150 µM) of extracellular pools of dNTPs 24 h post lincNMR depletion (n = 4). Data shown represent the results of the cell proliferation assay normalized to negative control siPOOL with the respective dNTP concentration. g Proposed model of lincNMR molecular mechanism. a–d, f Data shown are normalized to negative control siPOOL. Data represent mean, and error bars represent SEM. Significance was calculated by unpaired, two-tailed t test with *P < 0.05; **P < 0.01; ***P < 0.001.