Fig. 3: IMP-hydrolytic activity of PfISN1 and mutants at pH 8.0 and pH 5.0 and with ATP.
From: Structure and catalytic regulation of Plasmodium falciparum IMP specific nucleotidase
Histograms and error bars represent mean and SD, respectively, from three independent measurements. Location of mutations in PfISN1 is indicated above the histograms. v, the initial rate is the change in concentration of the product over time. NTRD, N-terminal regulatory domain. The concentration of enzyme and IMP used in all assays was 0.9 µM and 10 mM, respectively. Concentration of ATP was 2 mM. The conditions used for the assays are as mentioned in Supplementary Methods. WT, wild-type. Source data are provided as a Source data file.
