Fig. 2: Proteomic mapping of TMEM16K via in situ BioID-catalyzed biotin labeling finds endosomal transport as a major functional cluster.

a Scheme of proteomic mapping of protein complexes surrounding TMEM16K in the radius of 10 nm via in situ proximity biotinylation. b TMEM16K proteome candidate list is visualized as a protein-protein interaction network using the String protein interaction public database in Cytoscape. Candidates without known protein-protein interactions in the String database are represented on the bottom in the gray panel. TMEM16K is omitted from this representation for simplicity. Functional enrichment based on the GO terms was calculated using the String app in the Cytoscape and the major identified categories of functional enrichment were overlayed on our candidates with color-code. Purple: Endosomal transport (False Discovery Rate (FDR) p value = 2.49E−4), endosome to Golgi retrograde trafficking (FDR p value = 0.0096); Cyan: ER membrane protein complex (FDR p value=1.5E-5), protein localization to endoplasmic reticulum (FDR p value = 0.0026); Green: nuclear membrane (FDR p value = 1.28E−6), nuclear pore (FDR p value = 3.38E−9); Blue: proteasome (FDR p value = 5.74E−5). c Bioinformatic analysis of the TMEM16K candidates list with MCODE cluster app in Cytoscape identified major clusters in our dataset, which generated simplified network of TMEM16K proteomics data. Color coding of functional enrichment analysis was overlaid on the bioinformatically identified clusters. TMEM16K candidate list is provided in Supplementary Data 1.