Fig. 3: TMEM16K requirement for endosomal retrograde transport.
From: TMEM16K is an interorganelle regulator of endosomal sorting
a Left, Immunofluorescence of pulse chased antibody detecting CI-MPR internalized from the plasma membrane at 60 min time point in the WT, TMEM16K KO cell and TMEM16K KO cell with reintroduced TMEM16K. Scale bar 10 μm. Right, Ratio of measured intensity between vesicular region of the cell and the region encompassing Golgi (10 × 10 µm2). Single factor ANOVA, p value = 4.65E−37, post-test Bonferroni-corrected two sided t-test with pairwise comparison with WT (three biological replicates, n = 168 WT, 181 TMEM16K KO, p value = 1.37E−26***, and 134 KO + 16K cells). b Left, Immunofluorescence of Golgi marker GM130 and internalized conjugated cholera toxin B (CtxB) from the plasma membrane at 60 min time point in the WT, TMEM16K KO cell and TMEM16K KO cell with reintroduced TMEM16K. Scale bar 10 μm. b Right, Quantification of the Pearson’s correlation coefficient measuring colocalization of GM130 and CTxB. Single factor ANOVA, p value = 0.0018 with post-test Bonferroni-corrected two sided t-test with pairwise comparison with WT (three biological replicates, n = 40 WT, 57 TMEM16K KO, p value = 0.0051*, and 56 KO + 16K cells). In the box and whiskers plot, the box includes the first quartile and the third quartile, with the central line representing the median. Whiskers represent the minimum and maximum values of data. X inside the box represents the mean of data. Source data are provided as a Source Data file.
