Fig. 2: Super-resolution imaging of the lateral element protein SYCP3.
From: Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy
a SIM image of unexpanded SYCP3 labeled with Alexa Fluor 568. b dSTORM image of unexpanded SYCP3 labeled with Alexa Fluor 647. c Expanded MAP-SIM SYCP3 signal (maximum intensity projection) labeled with SeTau647. d–f Magnified views of boxed regions in a–c, respectively. The images indicate that MAP-SIM provides a similar labeling density and spatial resolution as dSTORM of 20–30 nm in the imaging plane. Scale bars, a–c 10 µm, d–f 3 µm.
