Fig. 3: 3D-multicolor MAP-SIM of SYCP3, SYCP1 N terminus, and SYCE3.
From: Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy
a SIM image of post-expansion SeTau647 labeled SYCP3 as a component of the lateral element (red), the transverse filament SYCP1 N terminus labeled with Alexa Fluor 488 (green), and SYCE3 of the central element labeled with Alexa Fluor 568 (magenta). b Magnified views of white boxed region in (a). ii Orthogonal view of horizontal white dashed line in i. iii Orthogonal view of vertical white dashed line in (i). c Transversal intensity profile perpendicular to the orientation of the SC shown in (b). The selected section exhibits a bimodal distribution of the SYCP3 signal separated by 667.0 nm ± 7.1 nm (SD). The SYCP1 N terminus and SYCE3 signals of the section (b) show monomodal distributions with FWHM of 214.7 ± 6.9 nm (SD) and 258.3 ± 4.8 nm (SD), respectively. d Large field of view (100 × 100 × 30 µm3) 3D-re-scan confocal microscopy image of spreadings. SYCP3 was labeled post-expansion with SeTau647. e Magnified view of boxed region in (d). (f) 3D-MAP-SIM image of the SCs of an entire set of chromosomes in a spermatocyte visualized by post-expansion labeling of SYCP3 with SeTau647. The inlet shows the enlarged view of the boxed region in (f). Scale bars, (a) 10 µm, (b), i–iii 1 µm, (d) 25 µm, (e) 10 µm, (f) 15 µm, (f), inlet 5 µm.
