Fig. 4: MAP-SIM reveals a complex organization of the SC central region.
From: Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy
a MAP-SIM image of SYCE3 labeled with Alexa Fluor 568 (magenta), SYCP1N labeled with Alexa Fluor 488 (green) and SYCP3 labeled with SeTau647 (red). Lateral view sections of the SC are visualized as twists in the SYCP3 signal (marked by white dotted boxes and indicated by arrows in the inlet of (a) showing only the SYCP3 channel). b Magnified view of white boxed region in (a. c, e, f) Enlarged views of highlighted region in (b) showing the SYCP3 signal in red (c), the SYCP1N signal in green (e) and the SYCE3 signal in magenta (f) with two sites selected for protein distribution analysis (1, 2). d Averaged profile of all cross-sectional intensity profiles along the SYCE3 (magenta) and SYCP1N (green) signals analyzed at regions specified in (a) by white dotted boxes showing a monomodal signal distribution of 168.0 ± 1.1 nm (SD) for SYCP1N and 211.43 ± 0.89 nm for SYCE3 derived from single Gaussian fitting. g Cross-sectional intensity profiles of lateral view sections 1 and 2 of the SC (indicated in e and f) show a multimodal organization of the central element protein SYCE3 (magenta) and the N terminus of SYCP1 (green). Scale bars, (a) 10 µm, (b) 3 µm, (c, e, f) 1 µm.
