Fig. 2: Naive/memory phenotype and immune checkpoint expression of T cells in TDLNs, and tumor. | Nature Communications

Fig. 2: Naive/memory phenotype and immune checkpoint expression of T cells in TDLNs, and tumor.

From: Tumor invasion in draining lymph nodes is associated with Treg accumulation in breast cancer patients

Fig. 2

Cell suspensions of TDLNs cells and tumors were stained for CD3, CD4, CD8, CD45RA, FOXP3, PD-1, CTLA-4, and ICOS. a Representative flow cytometric analysis (left panel) and frequency (right panel) of cells distinctively expressing CD45RA and/or FOXP3 among CD4+ T cells in TDLNs and tumor (Naive Treg: NI TDLN vs T p = 0.0023; I TDLN vs T p = 0.0004; Eff Treg: NI vs I TDLNs p = 0.0017; NI TDLN vs T p = 0.0004; I TDLN vs T p = 0.0009; naive Tconv: NI vs I TDLNs p = 0.0494; NI TDLN vs T p = 0.0002; I TDLN vs T p = 0.0001). Wilcoxon matched-pairs signed rank test. b–d Representative histograms and frequencies of b PD-1 (memory Tconv: NI vs I TDLNs p = 0.0436; NI TDLN vs T p = 0.0009; I TDLN vs T p = 0.0012; Foxp3+ non-Treg: NI vs I TDLNs p = 0.0203; NI TDLN vs T p = 0.0001; I TDLN vs T p = 0.0002; Eff Treg: NI vs I TDLNs p = 0.0031; NI TDLN vs T p = 0.0002; I TDLN vs T p = 0.0017), c CTLA-4 (memory Tconv: NI TDLN vs T p = 0.0023; I TDLN vs T p = 0.0004; Foxp3+ non-Treg: NI TDLN vs T p = 0.0031; I TDLN vs T p = 0.0004) and d ICOS (memory Tconv: NI vs I TDLNs p = 0.0107; FOXP3+ non-Treg: NI vs I TDLNs p = 0.0295; NI TDLN vs T p = 0.0009; I TDLN vs T p = 0.0245; Eff Treg: NI vs I TDLNs p = 0.0101; NI TDLN vs T p = 0.0436) expression among the indicated CD4+ T cell subpopulations. Wilcoxon matched-pairs signed rank test. e Representative flow cytometric analysis (upper panel) and frequency (lower panel) of ICOS and/or PD-1 among Eff Tregs (ICOS-PD-1+: NI TDLN vs T p = 0.0031; I TDLN vs T p = 0.0022; ICOS + PD-1+: NI vs I TDLNs p = 0.0215; NI TDLN vs T p = 0.0245; ICOS + PD-1-: NI TDLN vs T p = 0.0134; ICOS-PD-1-: NI vs I TDLNs p = 0.0023; NI TDLN vs T p = 0.0006). Wilcoxon matched-pairs signed rank test. f Heatmap displaying the frequency of expression of OX40, GITR, ICOS, CTLA-4, and PD-1 on the indicated T-cell subpopulations in TDLNs and tumor (N = 12). Red and blue indicate higher and lower expression frequencies, respectively. Wilcoxon matched-pairs signed rank test, *p < 0.05, **p < 0.01, ***p < 0.001.

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