Fig. 3: Astrocytic Ca2+ signals during sleep are most frequent in processes. | Nature Communications

Fig. 3: Astrocytic Ca2+ signals during sleep are most frequent in processes.

From: Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep

Fig. 3

a Representative ROA frequency heatmaps showing the localization of Ca2+ signals during single episodes of locomotion, whisking, quiet wakefulness, NREM sleep, IS sleep, and REM sleep states. Astrocytic somata outlined in black in the locomotion image. Scale bar 50 μm. b Representative image of GFAP-GCaMP6f fluorescence in astrocytes with ROIs over astrocytic somata (black) and neuropil, containing astrocytic processes (white circles of 10 μm in diameter) (left). Scale bar 30 μm. ROA frequency, expressed as number of ROAs per ROI per minute, in astrocyte somata and processes from ROIs shown in left across sleep-wake states (right). Data represented as estimates ± SE and p-values (two-sided test, no adjustment for multiple comparisons) derived from linear mixed effects models statistics, n = 6 mice, 247 trials. For details on statistical analyses, see “Methods.” See also Supplementary Fig. 11.

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