Fig. 8: Astrocytic IP3-mediated Ca2+ signaling pathway regulates SWS. | Nature Communications

Fig. 8: Astrocytic IP3-mediated Ca2+ signaling pathway regulates SWS.

From: Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep

Fig. 8

a–c Bout duration (a), bouts per hour (b), and percentage recording time (c) of NREM, IS, and REM sleep in WT and Itpr2−/− mice. For WT mice: n = 3 mice, 806 NREM episodes, 617 IS episodes, 92 REM episodes; for Itpr2−/− mice: n = 3 mice, 1140 NREM episodes, 787 IS episodes, 86 REM episodes. d Awakening probability calculated as awakenings from NREM, IS, or REM sleep divided by total number of NREM, IS, or REM episodes. For WT mice: n = 4 mice, 806 NREM episodes, 617 IS episodes, 92 REM episodes; for Itpr2−/− mice: n = 3 mice, 1140 NREM episodes, 787 IS episodes, 86 REM episodes. e Mean ± SEM of astrocytic Ca2+ signals during transitions from NREM, IS, or REM sleep to wakefulness. For WT mice: n = 6 mice, 59 NREM awakenings, 141 IS awakenings, 87 REM awakenings; for Itpr2−/− mice: n = 6 mice, 23 NREM awakenings, 56 IS awakenings, 27 REM awakenings. f Frequency of microarousals during NREM sleep. For WT mice: n = 6 mice, 355 microarousals; for Itpr2−/− mice: n = 6 mice, 240 microarousals. g Mean ± SEM of (top to bottom) astrocytic Ca2+ signals, z-scores of EMG activation and whisker motion aligned to the beginning of a microarousal. For WT mice: n = 6 mice, 303 microarousals; for Itpr2−/− mice: n = 5 mice, 191 microarousals. h Number of NREM-to-IS, IS-to-NREM, and IS-to-REM transitions. For WT mice: n = 4 mice, 386 NREM-to-IS, 187 IS-to-NREM, 68 IS-to-REM transitions; for Itpr2−/− mice: n = 3 mice, 440 NREM-to-IS, 267 IS-to-NREM, 61 IS-to-REM transitions. i Mean ± SEM of astrocytic Ca2+ signals during NREM-to-IS, IS-to-NREM, and IS-to-REM transitions in WT and Itpr2−/− mice. For WT mice: n = 6 mice, 90 NREM-to-IS, 35 IS-to-NREM, 25 IS-to-REM transitions; for Itpr2−/− mice: n = 6 mice, 62 NREM-to-IS, 14 IS-to-NREM, 8 IS-to-REM transitions. Data represented as estimates ± SE and p-values (two-sided test, no adjustment for multiple comparisons) derived from linear regression models unless otherwise stated. For details on statistical analyses, see “Methods”.

Back to article page