Fig. 1: MP CD4+ T lymphocytes contain three subsets based on T-bet expression. | Nature Communications

Fig. 1: MP CD4+ T lymphocytes contain three subsets based on T-bet expression.

From: Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state

Fig. 1

(a) Definition of MP cells. The representative dot plot shows CD44 and CD62L expression by Foxp3−CD4+ αβT lymphocytes in spleen from five mice. (b and c) T-bet and RORγt expression in MP and naïve CD4+ T lymphocytes. The representative plots show (b) T-bet-AmCyan and RORγt-E2Crimson expression in the indicated cell populations obtained from three T-bet-AmCyan RORγt-E2Crimson double reporter mice and (c) T-bet and CXCR3 protein levels in the same populations from five mice. (d–f) T-bethigh MP cells produce IFN-γ in the absence of TCR signaling in Toxoplasma-infected mice. d Experimental design. CD4CreERT2 TCRαflox IFN-γ-YFP T-bet-AmCyan mice receiving TMX on Days -10 and -8 were infected with T. gondii on Day 0 and splenocytes measured for IFN-γ-YFP expression in T-bethigh, T-betlow, and T-bet−TCRβ− MP subsets several days later. e TCRβ expression in MP cells and T-bet-AmCyan levels in their TCRβ− fraction on Day 0. f IFN-γ-YFP expression in T-bethigh, T-betlow, and T-bet−TCRβ− MP cells on Days 0, 2, and 8 following T. gondii infection. A representative histogram is shown (orange, purple, and red lines indicate YFP expression by T-bethighTCRβ− MP cells on Days 0, 2, and 8, respectively) while the graph depicts the MFI (mean ± SD) of IFN-γ-YFP expression in the indicated populations (red T-bethighTCRβ− MP; green T-betlowTCRβ− MP; blue T-bet−TCRβ− MP; gray TCRβ− Naïve; Day 0 n = 6 mice; Day 2 n = 3 mice; Day 8 n = 3 mice). Data are representative of two independent experiments performed. (g and h) T-bethigh MP cells can prolong survival in T. gondii infection. g Experimental design. Rag2 / Il2rg DKO mice were infected with T. gondii on Day 0, received sorted T-bethigh, T-betlow, or T-bet− MP cells on the next day, and were monitored for survival. h Survival of T. gondii-infected Rag2 / Il2rg DKO mice that received each MP subset (red, green, and blue lines show T-bethigh, T-betlow, and T-bet− MP cells, respectively; T-bethigh MP n = 5 mice; T-betlow MP n = 5 mice; T-bet− MP n = 5 mice; Untransferred controls n = 9 mice). Data are pooled from two experiments performed. (f) A two-sided t and (h) a log-rank tests were performed to assess significance. Source data are provided as a Source Data file.

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