Fig. 4: Extracted parameters of α₃D folding from 3c-PIE and 3c-CW.

a Relaxation rate k (= k_F + k_U) from 3c-PIE (blue) and 3c-CW (red). b Fraction of the folded state p_F from 3c-PIE (blue) and 3c-CW (red). c Folded state FRET efficiency E_1F from two color (black), 3c-PIE (blue), and 3c-CW (red). d Unfolded state FRET efficiency E_1U from two color (black), 3c-PIE (blue), 3c-CW (red), and E_1U^3c calculated using the Gaussian chain model with parameters obtained from two-color FRET efficiency for 3c-CW (green) and 3c-PIE (purple) (see Methods). In c, d, E₁^3c values of 3c-CW were determined using E₂^2c from two-color (DA2) segments. E₁^3c values of 3c-PIE were calculated using E₁₂^2c obtained from A1 excitation. e FRET efficiencies between A1 and A2 in the folded (E_12F^2c) and unfolded (E_12U^2c) states in 3c-CW using two-color E₂^2c (red), the unfolded state FRET efficiency calculated using the Gaussian chain model (green), and E_12F^2c and E_12U^2c determined from 3c-PIE by A1 excitation (blue). Error bars are standard deviations calculated from the curvature at the maximum of the likelihood function. Source data are provided as a Source Data file.