Fig. 6: Determination of the relaxation rate and population for recolored photon trajectories.
From: Fast three-color single-molecule FRET using statistical inference
See Supplementary Fig. 10 for the acceptor fractions. a α3D folding. Experimental photon trajectories collected at three different GdmCl concentrations (blue, 2 M, pF = 0.3; red, 2.25 M, pF = 0.47; yellow, 2.5 M, pF = 0.63) were recolored using the corresponding maximum likelihood parameters except the relaxation rate (kF + kU, ksim) that was varied in the simulation. b TAD/NCBD binding. Experimental photon trajectories at 0 mM NaCl were recolored using the extracted maximum likelihood parameters at 0 mM NaCl except the relaxation rate (kB + kU, ksim) and bound fraction (pB,sim, blue, 0.3; red, 0.5; yellow, 0.7) that were varied in the simulation. n is the average photon count rate of the three-color segments, which are ~80 ms−1 for α3D folding and 65 ms-1 for TAD/NCBD binding. Horizontal dashed lines in pF and pB indicate the values in simulation. Error bars are standard deviations of the parameters obtained from five recolored data set. Source data are provided as a Source Data file.
