Fig. 4: Protective efficacy of LmCen−/− parasites against virulent L. major needle challenge in C57BL/6 mice. | Nature Communications

Fig. 4: Protective efficacy of LmCen−/− parasites against virulent L. major needle challenge in C57BL/6 mice.

From: A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing

Fig. 4

a Schematic representation of the needle challenge procedure. b Ear lesion diameter was measured weekly for both LmCen−/− immunized (Imm Chal) (n = 13) and non-immunized (Non-Imm Chal) (n = 13) mice after intradermal challenge with LmWT parasites. Results are represented as SEM, of 1 ear from each of the 13 mice per group and data pooled from two independent experiments. Statistical analysis was performed by unpaired two-tailed t-test (***p-0.0005, ****p < 0.0001). c Photographs (left panel) & histology (H&E stained, right panel) of representative ear of age-matched naïve, LmCen−/− immunized (Imm Chal) & non- immunized (Non-Imm Chal) mice after 10 weeks post challenge from one of two independent experiments (n = 2). Ear lesion histology sections are representative of one mouse from each group of  one of two independent experiments. Arrow indicates inflammatory cells recruited area. Scale Bars: 100 μm. d and e, Scatter dot plots of parasite load in the challenged ears (d) and in the draining lymph nodes (e) of each LmCen−/− immunized (Imm Chal) (n = 13) & non-Immunized (Non-Imm chal) (n = 13) mice. Parasite burden was determined by limiting dilution assay. Results are mean ± SEM. Data are pooled from two independent experiments. Statistical analysis was performed by unpaired two-tailed t-test (**p -0.004, ***p-0.0004).

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