Fig. 3: Histological analysis of the tumorigenicity study.

a Survival curves of NOG mice tested in the tumorigenicity study. b–e Representative images of brain sections of the NOG mice used in the tumorigenicity study. b–d H–E staining (c is a magnification of b, and d a magnification of c) and e immunohistochemistry for tyrosine hydroxylase counter stained by hematoxylin. Bars in b = 1 mm, c = 500 μm, and d, e = 100 μm. Number of cell preparation = 6, and number of animals = 80 (transplantation) and 50 (control). f Time course of the subcutaneous mass volume of NOG mice used in the teratoma formation assay. Dots are mean values of each group. g–o Representative images of grafts in the subcutaneous space of NOG mice injected with HeLa cells (g, number of animals = 8), 201B7 iPSCs (h, i, number of animals = 6), MCB003 iPSCs (j–l, number of animals = 20), and MCB003 DAPs (m–o, number of animals = 21). i is a magnification of h, k, l are magnifications of j, and n, o are magnifications of m. l, o Staining by KU80. Bars in g, h, j, m = 1 mm, in i, k, l, n, o = 100 μm. g–i Number of cell preparation = 1 and j–o number of cell preparations = 7.