Fig. 2: Extending the canonical THIQ pathway to (S)-reticuline.
From: A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
a Composite pathway for synthesis of (S)-reticuline (11) from (S)-norcoclaurine (3). b Relative titers of (S)-reticuline pathway intermediates in culture supernatants of successive engineered production strains. Peak area counts are shown for all pathway intermediates and absolute titers are depicted in mg L–1 for (S)-norcoclaurine and (S)-reticuline. Error bars represent the mean ± s.d. of n = 3 independent biological samples. Asterisk (*) denotes a significant increase or decrease (P < 0.05) in (S)-norcoclaurine or (S)-reticuline titer relative to the parental strain. Statistical differences between control and derivative strains were tested using two-tailed Student’s t-test. c Cultivation of an (S)-reticuline-producing strain (LP507 harboring pHUM) in a sucrose-pulsed fed-batch fermentor using a simple mineral medium. Growth of biomass (OD600) and accumulation of BIA metabolites in the culture medium were measured during cultivation. Data points from duplicate experiments are shown and the mean is depicted as a line. Source data underlying Fig. 2b, c are provided in a Source Data file.
