Fig. 7: Detailed base interactions in the PET-PTC components upon transition from state A to state B/C.
From: Structural snapshots of human pre-60S ribosomal particles before and after nuclear export
a–e Selected rRNA residues of the PTC-PET components in the immature state A is shown in stick models, superimposed with local densities. Residues from H90, H73-H93 linker, H89, H92, H73-H74 linker are colored yellow, purple, cyan, pink and blue, respectively. Non-native (immature conformation) base-pairings between C4453 (H90) and G4527 (H90), between G4448 (H89) and C4502 (H92), and between A4449 (H89) and U4501 (H92) are highlighted in a and c. f–j Same regions of mature -like state C as in a–e are shown. Base flip of G4527 (f) in state C (mature like) disrupts the base-pair in a. Native base stacking formed in state C between G4527 (H90) and U4556 (H73-H93 linker) is shown in g. Multiple base stacking interactions formed between residues of H89 and H73-H74 linker are highlighted in i, j.
