Fig. 3: A fraction of the genome harbours methylated H3K4 and/or H3K27 at the same location in most sperm of a population. | Nature Communications

Fig. 3: A fraction of the genome harbours methylated H3K4 and/or H3K27 at the same location in most sperm of a population.

From: Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription

Fig. 3

a Percentage of the genome with different range of apparent histone H3 methylation density (HMD) on Lysine 4 and 27 in Xenopus Laevis sperm and mouse ESC. b Percentage of H3K4me3 and H3K27me3 peaks with different range of HMD in Xenopus Laevis sperm. c Fold enrichment (observed/random) over 1000 randomisations of peaks with homogeneous histone methylation (HMD > 80) at the indicated genomic features; ***: empirical p value < 1e−3. ICe-ChIP data from two independent replicates were pooled. d Dot matrix showing transcription factors with enriched motifs (y-axis) in the different histone methylation categories (x-axis). Circle size represents –log10 (p value) of the motif enrichment; and the circle colour indicates whether evidences exist indicating that the corresponding transcription factors is present maternally (blue) or not (red). Retained HMD > 80 peaks correspond to sperm histone methylation peaks maintained after extract treatment as in Fig. 5. e Heat map after PAM clustering of promoters (TSS +/−2 kb) according to histone H3-methylation density on Lysine 4 and 27. The plots on the left show the average HMD profile for each cluster. Source data related to a, b, d, and e are provided as Source Data files.

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