Fig. 6: The homogeneously methylated histone fraction in frog sperm chromatin regulates embryonic gene expression.
a Barplot of enrichment p values (−log10) in set of genes expressed at zygotic gene activation (all-, developmental- and house keeping-ZGA genes) for the presence of different type of H3K4me3 or H3K27me3 sperm peaks. pink and light green bars = enrichment p value for heterogenous (HMD < 80) peaks in promoter; red and green bars = enrichment p value for homogeneous (HMD > 80) peaks in promoter that are also retained after replication. P values determined by the χ2 proportion test evaluating if the proportion of gene of interest is higher (alternative = ‘greater’) than the genome-wide proportion (i.e., expected proportion). b Barplot of enrichment p values (−log10) in set of genes whose embryonic expression is sensitive to the presence of histone H3K4 demethylase (Kdm5b) or histone H3K27 demethylase (Kdm6b) at fertilisation2 for the presence of the aforementioned sperm peaks categories. P values are obtained as in a. c Pie chart indicating the percentage of the homogeneously methylated histone present in sperm that are retained after egg extract-mediated replication. Three type of sperm peaks are considered: homogeneous for H3K27me3 only, homogeneous for H3K4me3 only and homogeneous for H3K27me3 and H3K4me3 (bivalent). The area of the coloured sector (together with the colour) indicates the fraction of the overlap between each pair-wise comparison. d Barplot of enrichment p values (−log10) in various set of genes for the presence of a homogeneous bivalent H3K4me3 and H3K27me3 retained after replication. P values are obtained as in a. HMD are from data pooled from two independent replicates. Peaks retention/lost are consensus from three independent replicates. Set of kdm5 and kdm6 sensistivite genes are from three independent replicates.
