Fig. 5: PRDM10 transcriptionally regulates Eif3b expression.
From: Global translation during early development depends on the essential transcription factor PRDM10
a ChIP-seq signal tracks showing PRDM10 occupancy at the Eif3b promoter in EtOH- vs. OHT-treated Prdm10F/F; CreERT2 mESCs, analyzed at 2 days post-induction. Vertical axis: fold-change over input. b PRDM10 enrichment at the Eif3b promoter is detected as two subpeaks, denoted P1 and P2 (left). PRDM10-dependent activation of luciferase reporters containing Eif3b promoter sequences derived from subpeak P1 or P2 (right); n = 2 samples. Representative data shown from one out of three independent experiments. c qRT-PCR quantification of changes in Eif3b transcript at indicated time-points after Prdm10 deletion; n = 2–3 biological replicates. d Western blot analysis of EIF3B protein levels in Prdm10F/F; CreERT2 mESCs at indicated time-points after treatment with EtOH (E) or 4-OHT (O). e qRT-PCR analysis showing restoration of Eif3b transcript levels in Prdm10Δ/Δ mESCs by exogenous Prdm10. Data shown at Day 4 post-induction; n = 3. Gene expression shown relative to Ubb (c and e). Error bars denote mean ± s.d.; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s., not significant; two-tailed unpaired Student’s t test (b, c and e).
