Fig. 1: MAIT cell alterations during obesity. | Nature Communications

Fig. 1: MAIT cell alterations during obesity.

From: Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity

Fig. 1

a Representative dot plots of MAIT cell and control MR1-Tet staining. The numbers represent MAIT cells frequency among αβT cells. b MAIT cell frequency among αβT lymphocytes in blood (ND n = 6; HFD n = 6), liver, colon, ileum (ND n = 5; HFD n = 6), Epi-AT (ND n = 6; HFD n = 7), and spleen (n = 10 per group). c MAIT cell absolute numbers in the ileum (ND n = 8; HFD n = 10) and MAIT cell number per gram of Epi-AT (ND n = 13; HFD n = 12). d MAIT cell frequency kinetic analysis of mice fed ND or HFD in the ileum (0 weeks n = 8; 6 weeks ND n = 11, HFD n = 14; 12 weeks ND n = 9, HFD n = 10; 16 weeks ND n = 10, HFD n = 13) and Epi-AT (0 weeks n = 8; 6 weeks ND n = 11, HFD n = 14; 12 weeks ND n = 15, HFD n = 15; 16 weeks ND n = 17, HFD n = 19). Pooled data from four independent experiments are represented. e Migration of MAIT cells into lean or obese recipient mice after 12 weeks of ND or HFD. Purified MAIT cells from CD45.1 Vα19 transgenic Cα−/− mice were transferred into CD45.2 mice and analyzed 5 days later. Graphs represent CD45.1 MAIT cells frequency in the ileum (n = 10 per group) and Epi-AT (n = 10 per group). Pooled data from two independent experiments are represented. f Ki67 staining of MAIT cells from the ileum (ND n = 8; HFD n = 10) and Epi-AT (ND n = 10; HFD n = 10) of 12 weeks HFD or ND-fed mice. Pooled data from two independent experiments are represented. g qPCR analysis of apoptotic (cMyc, Caspase9, and Bax) and anti-apoptotic (Bcl-2) gene expression by FACS-sorted MAIT cells from mice fed ND (n = 8) or HFD (n = 9) for 12 weeks. Pooled data from two independent experiments are represented. h BCL-2 MFI in MAIT cells from the ileum and Epi-AT from mice fed ND or HFD for 12 weeks (n = 4 per group). For b, c, e, and f, each symbol represents an individual mouse (small horizontal lines indicate the mean ± S.E.M.). In d, g, and h, data are represented as mean ± S.E.M. Statistical analysis were performed by two-tailed Mann–Whitney test, *P < 0.05, **P < 0.01, ***P < 0.001 (see also Supplementary Fig. 1).

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