Fig. 2: MAIT cell phenotype and function during obesity. | Nature Communications

Fig. 2: MAIT cell phenotype and function during obesity.

From: Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity

Fig. 2

a, b MAIT cell frequency kinetic analysis of B6 mice fed ND or HFD for 3, 6, and 12 weeks. a Graphs representing CD44 mean fluorescence intensity (MFI) (3 weeks ND n = 5, HFD = 6; 6 weeks ND n = 8, HFD n = 9; 12 weeks ND n = 9, HFD n = 11) and percentage of CD69+ MAIT cells (3 weeks ND n = 8, HFD = 9; 6 weeks ND n = 7, HFD n = 11; 12 weeks ND n = 8, HFD n = 9) among the total MAIT cells from the ileum. Pooled data from three independent experiments are represented. b Graphs representing CD44 mean fluorescence intensity (MFI) (3 weeks ND n = 5, HFD = 6; 6 weeks ND n = 9, HFD n = 12; 12 weeks ND n = 7, HFD n = 8) and percentage of CD69+ MAIT cells 3 weeks ND n = 10, HFD = 10; 6 weeks ND n = 9, HFD n = 12; 12 weeks ND n = 8, HFD n = 10) among total MAIT cells from Epi-AT. Pooled data from three independent experiments are represented. Representative histograms of staining on MAIT cells from the ileum and Epi-AT. c Intracellular staining of MAIT cells for TNFα, IFN-γ, and IL-17A. Frequency of positive MAIT cells from the ileum and Epi-AT of lean (n = 8) and obese (n = 9) mice are indicated. Pooled data from three independent experiments are represented. d Analysis of the abundance of MAIT cell-activating ligands using a bioassay based on the activation of purified MAIT cells by fecal supernatants from the cecum of HFD-fed (n = 14) mice or ND-fed (n = 15) mice in the presence of Ac-6-FP when indicated. Pooled data from four experiments are represented. e Heatmap representing microbial gene level abundance of key riboflavin biosynthesis enzymes from ND-fed mice and 12 weeks HFD-fed mice (n = 20 for per group). f The Riboflavin biosynthesis pathway in green decreased enzyme gene expression, and in red, increased enzyme gene expression. For a–d, each symbol represents an individual mouse (small horizontal lines indicate the mean ± S.E.M.). Statistical analysis was performed by two-tailed Mann–Whitney test, *P < 0.05, **P < 0.01, ***P < 0.001 (see also Supplementary Figs. 2–4).

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