Fig. 4: MAIT cells impact other immune cell populations in Epi-AT of obese mice and induce inflammation. | Nature Communications

Fig. 4: MAIT cells impact other immune cell populations in Epi-AT of obese mice and induce inflammation.

From: Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity

Fig. 4

a Graph showing the relative quantity of pro- and anti-inflammatory cytokine transcripts in Epi-AT of Vα19+/− and MR1−/− mice and their littermate controls fed with HFD during 12 weeks (n = 6 mice per group). b–d Graphs showing the frequency of Foxp3+ Treg cells, ILC2, eosinophils (b), CD8 αβT cells, NK cells (c), and macrophages (d) among CD45+ cells in the Epi-AT stroma-vascular fraction (SVF) of B6 mice fed with ND (n = 10) or HFD (n = 10), and Vα19+/− (ND n = 12, HFD n = 13) and MR1−/− (ND n = 10, HFD n = 16) mice and their respective littermate controls (Vα19−/− (ND n = 12, HFD n = 11) and MR1+/− (ND n = 12, HFD n = 17)) after 12–16 weeks of HFD or ND feeding. Pooled data from two to four experiments are represented. e Representative dot plot of Epi-AT macrophages sub-populations in Vα19+/− and MR1−/− mice and their respective littermate controls after 12–16 weeks of HFD or ND feeding. Data show the frequency of M2 (CD206+CD11c) and M1 (CD206−CD11c+) among CD11b+F4/80+ total macrophages. f M1 (CD206−CD11c+) and M2 (CD206+CD11c+) ratio in the Epi-AT of Vα19+/− (n = 5), MR1−/− (n = 6) and their littermate controls (Vα19−/− n = 5 and MR1+/− n = 6). g Graphs showing the relative quantity of pro-inflammatory M1 markers (Ccr2, Mcp1, Il1β, Il6, and Tnfα) and anti-inflammatory M2 markers (Tgfbr2, Cd206, Cd163, and Il10) transcripts in Epi-AT macrophages from Vα19+/− (n = 10) and MR1−/− (n = 14) mice and their respective littermate controls (Vα19−/− n = 13 and MR1+/− n = 16) fed on HFD during 12 weeks. h Kinetic analysis of M2 macrophages and M1-macrophages frequency of Vα19+/− and MR1−/− mice and their respective littermate controls fed with ND or HFD during 3, 6, and 12 weeks (n = 6 per group for each time point). In a, e, and h, all data are represented as mean ± S.E.M. For b–d and g, each symbol represents an individual mouse (small horizontal lines indicate the mean ± S.E.M.). All statistical analyses were performed by two-tailed Mann–Whitney test. *P < 0.05, **P < 0.01, ***P < 0.001 (see also Supplementary Figs. 7, 8, 9 and 10).

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