Fig. 7: MAIT cells impact on microbiota, gut mucosa and adipose tissue fecal transplanted or co-housed mice. | Nature Communications

Fig. 7: MAIT cells impact on microbiota, gut mucosa and adipose tissue fecal transplanted or co-housed mice.

From: Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity

Fig. 7

a Flowchart illustrating feces transfer procedure and data-analysis workflow. b Heatmap showing the significant differences in OTU clusters abundance between recipient mice T-MR1+/− that received microbiota from obese MR1+/− mice and the T-MR1−/− mice that received microbiota from obese MR1−/−. c Significant differences at the genus level between the microbiota of recipient mice T-Vα19−/− (n = 12) (microbiota from obese Vα19−/− mice) and the T-Vα19+/− (n = 11) (microbiota from obese Vα19+/−). d Heatmap showing the significant differences between the OTU clusters of the T-Vα19−/− and T-Vα19+/− mice microbiota. e Intestinal permeability measured by FITC-dextran assay (n = 6/group). f, g Graphs showing the frequency of immune cells in the ileum (f) and Epi-AT (g) of T-Vα19−/− (n = 10), T-Vα19+/− (n = 10), T-MR1+/− (n = 10) and T-MR1−/− (n = 10). h Graphs showing the frequency of Foxp3+ Treg cells and ILC3 among CD45+ cells in the ileum lamina propria (n = 9 mice/group). i Graphs showing the frequency of eosinophils among CD45+ cells in the Epi-AT stroma-vascular fraction (SVF) (n = 9 mice/group). j Graphs showing the frequency of total Epi-AT macrophages among CD45+, of M2 and M1 subsets among total Epi-AT macrophages and M1/M2 macrophage ratio (n = 9 mice/group. k Graphs showing the relative quantity of pro-inflammatory M1 markers (Ccr2, Mcp1, Il1β, Il6, and Tnfα) and anti-inflammatory M2 markers (Tgfbr2, Cd206, Cd163, and Il10) transcripts in Epi-AT macrophages from Vα19+/− (n = 6) and MR1−/− (n = 6) mice co-housed with their respective littermate controls (Vα19−/− n = 6 and MR1+/− n = 5) and fed on HFD during 12 weeks. Data in d are presented as boxplots, as mean ± S.E.M.. For e, f, and h–j, each symbol represents an individual mouse (small horizontal lines indicate the mean ± S.E.M.). In k, data are represented as mean ± S.E.M.. All statistical analyses were performed by two-tailed Mann–Whitney test. *P < 0.05, **P < 0.01, ***P < 0.001 (see also Supplementary Fig. 14).

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