Fig. 2: Risk allele sensitizes the enhancer to androgens.

a The graph depicts relative luciferase activity over Renilla for the empty plasmid and the plasmid harboring the rs72725854 enhancer region in LNCaP cells. b The graph depicts the fold change in reporter activity of empty plasmid, “G” and “T” allele over “A” allele in LNCaP cells. Error bars denote SEM from multiple biological replicates (n > 3) in a and b. c UCSC genome browser snapshot around the rs72725854 region with ChIP-seq tracks for AR in tumors, healthy individuals and LNCaP cells and, FOXA1 in tumors and LNCaP cells. d The graph depicts the relative change over Renilla for the empty plasmid and plasmids harboring the different alleles “A”, “G”, and “T” of rs72725854 enhancer in LNCaP cells grown in charcoal stripped media for three days including the treatment with methanol or 10 nM DHT for 16 h. Error bars denote SEM from multiple biological replicates (n > 3). e q-RT PCR analysis showing eRNA expression at rs72725854-harboring enhancer upon 10 nM DHT treatment for 16 h in LNCaP cells. Error bars denote SEM from three biological replicates. p-values were calculated by Student’s two-tailed unpaired t-test in a, b, d, and e ****p < 0.0001, ^nsp > 0.05. Source data are provided as a Source Data file.