Fig. 4: Revealing gene expression identity of intratumoral Cyp11a1+ T cells by scRNAseq.
From: Tumors induce de novo steroid biosynthesis in T cells to evade immunity
a, b UMAP visualization of the tumor-infiltrating T cells (a) with annotations of the clusters (b). Tc1 represents type-1 CD8+ T cells, Tc2 represents type-2 CD8+ T cells, and Th2 represents type-2 CD4+ T cells. c, d mCherry protein expression accurately reports Cyp11a1 mRNA expression. Cyp11a1-mCherry protein expression according to the flow cytometry (FACS) data (c). Cyp11a1 mRNA expression in the scRNA-seq data (d). e Expression of cell-type-specific signature genes that were used to annotate the clusters. f Determination of Cyp11a1 locus control region. Open chromatin regions were identified by ATAC-seq of T helper cells. In vitro generated Th2 and Th17 cells were used as positive control. Naïve and Th1 cells were used as negative control. Gata3 ChIP-seq data were analyzed to determine the binding site. The open chromatin region where Gata3 occupy is highlighted blue and considered as locus control region.
