Fig. 5: Ablation of T cell steroidogenesis restricts experimental tumor growth and metastasis.
From: Tumors induce de novo steroid biosynthesis in T cells to evade immunity
a Left-panel: B16-F10 subcutaneous tumor growth curve assessed in T cell-specific Cyp11a1 cKO (cKO) and Cd4-Cre control mice. N = 5. Representative of four independent experiments. Right panel: graphical presentation of end-point tumor volume. N = 20 (ctrl), 23 (cKO). Pooled data of four independent experiments. Each point represents an individual animal. Error bars represent mean ± s.e.m., unpaired two-tailed t-test. b Left panel: Representative photograph of pulmonary metastatic foci produced 10 days after tail vein injection of B16-F10 cells in cKO and control (Cre and WT) mice. N = 5. Right panel: graphical presentation of the numbers of lung metastatic foci. N = 14 (ctrl), 16 (cKO), pooled data of three independent experiments, each point represents an individual animal, error bars represent mean with s.d. Unpaired two-tailed t-test. c cKO and Cd4-Cre control mice were injected with B16-F10 cells. Pregnenolone or vehicle (DMSO) applied topically at the primary tumor site every 48 h. Tumor volume was measured at the end-point at day 12. N = 5. Each point represents an individual animal. Error bars represent mean ± s.e.m. P-value was calculated by one-way ANOVA with Tukey Post hoc test. d Representative hematoxylin and eosin stained histologic photograph of pulmonary metastatic foci produced 10 days after tail vein injection of EO771 cells in Cyp11a1 cKO and Cyp11a1fl/fl mice. Graphical presentation of the numbers of lung metastatic foci (right panel). N = 10. Error bars represents mean with s.d. Unpaired two-tailed t-test. Representative of two independent experiments. e B16-F10 cells were injected subcutaneously in C57BL/6 mice with or without Cyp11a1 inhibitor aminoglutethimide (AG). AG treatment was continued with a 48-h interval. N = 5. Error bars represent mean ± s.e.m. Two-way ANOVA. Representative of two independent experiments. f EO771 cells were injected into the mammary fat pad in C57BL/6 mice with or without Cyp11a1 inhibitor AG. AG treatment was continued with a 48-h interval. N = 5. Error bars represent mean ± s.e.m. One experiment. In this figure, N represents biologically independent animals.
