Fig. 3: The linker region upstream of HR2 motif important for membrane fusion.
From: Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein
a Binding of the linker region upstream of HR2 motif along the central helix colored as in Fig. 1a. b The electrostatic surface representation of the linker region upstream of HR2 motif binding area. Red represents negative charge, and blue represents positive charge. c Overall structural changes of the linker region upstream of the HR2 motif during membrane fusion. The linker regions in pre-fusion20 (PDB: 6NB6) and post-fusion states are shown as cartoons colored in violet and green, respectively. The SD3 domains in pre-fusion20 (PDB: 6NB6) and post-fusion states are shown as cartoons colored in pale cyan and orange, respectively. d Structural changes of the linker region upstream of the HR2 motif during membrane fusion represented in single chain mode. The cartoons are colored as in (c). e Structural details of the linker region and the SD3 domain from the pre-fusion SARS-CoV20 (PDB: 6NB6) colored as in (c). f Structural details of the linker region and the SD3 domain from the post-fusion SARS-CoV colored as in (c). The disulfide bond is shown as spheres.
