Fig. 1: MIWI2 interacts with TEX15 in gonocytes undergoing de novo genome methylation.
From: TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
a HA stained Miwi2HA/+ E16.5 testes section untreated or treated with RNase A (n = 3). DNA stained with DAPI. Scale bar 1 µm. b Western blot of HA-MIWI2 from pellet (P) and soluble fraction (S) of Miwi2HA/HA E16.5 testis lysates prepared with or without RNase A (n = 3). The ratio of HA-MIWI2 in the soluble and pellet fractions is shown, samples were processed in parallel and ratio between S and P acts as internal control. c, d Volcano plots showing enrichment (log2(mean LFQ ratio HA IP Miwi2HA/+ per control HA IP Miwi2+/+) over statistical confidence (−log10(P value of two-sided Student’s t-test)) of proteins co-immunoprecipitated with HA-MIWI2 from E16.5 testes lysates (c) untreated, (d) RNase A treated during protein extraction (n = 3). Highlighted proteins indicate >4-fold enrichment and significance P < 0.05. e Schematic representation of TEX15 domains and the nuclear localisation signal (NLS) prediction. f HA stained Tex15HA/HA E16.5 testis section (n = 5). DNA stained with DAPI. Scale bar 10 µm. Bottom left panel shows close up of highlighted gonocyte. Scale bar 5 µm.
