Fig. 7: Salmonella increases lactate production by inflammatory BM neutrophils. | Nature Communications

Fig. 7: Salmonella increases lactate production by inflammatory BM neutrophils.

From: Lactate released by inflammatory bone marrow neutrophils induces their mobilization via endothelial GPR81 signaling

Fig. 7

a, b Lactate levels in (a) BM or (b) blood from WT mice treated with PBS or Salmonella (n = 5). a ***p(0.0004); b **p(0.0081). c Quantitative analysis of ROS production in BM neutrophils from WT (n = 7) vs. gp91phox−/− (n = 6) mice infected with Salmonella. d Percentage of BM HIF-1α+ neutrophils in WT (n = 9) vs. gp91phox−/− (n = 9). e Quantitative analysis and representative flow cytometry histogram plot of LDHA expression in BM neutrophils from WT (n = 7, PBS; n = 6, Salmonella; ***p(0.0001)) vs. gp91phox−/− (n = 6, PBS; n = 5, Salmonella) mice. f Quantitative analysis of MCT4 expression on BM neutrophils from WT (n = 6; ***p(0.0008)) and gp91phox−/− (n = 5) mice. g Quantitative analysis and representative flow cytometry histogram plot of ROS production in PB neutrophils from WT (n = 5; **p(0.0030)) vs. gp91phox−/− (n = 7, PBS; n = 6, Salmonella) mice. h Frequency of PB neutrophils from WT (n = 7, PBS; n = 6, Salmonella) vs. gp91phox−/− (n = 7) and GPR81−/− (n = 8) mice. i Frequency of liver neutrophils from WT (n = 7, PBS; n = 6, Salmonella) vs. gp91phox−/− (n = 7) and GPR81−/− (n = 8) mice. Data are represented as mean ± SEM from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001, Student’s two-tailed unpaired t test (a, b), two-way ANOVA with Tukey’s post hoc test (c–i).

Back to article page