Fig. 9: PEAK1 expression regulates tumor growth and metastasis of TNBC in vivo.

a Lysates of MDA-MB-231-Luc CRISPR PEAK1 knockout clones. b Representative in vivo bioluminescent images of mammary fat pad-injected mice with MDA-MB-231-Luc WT (parental) or CRISPR PEAK1 KO cells 4 weeks postinjection. c Bioluminescence quantification of tumor growth at different weeks postinjection of mice bearing parental (n = 5), PEAK1 KO1 (n = 5), or PEAK1 KO2 (n = 5). P < 0.0001. d Representative bioluminescent lung images of mice shown in b. For the lungs of the mice bearing CRISPR PEAK1 KO tumors, lungs were dissected once the tumor reached the size of the WT tumors. e Circulating tumor cells isolated from mice-bearing parental or PEAK1 KO mammary tumors. ***P = 0.000022; ****P = 0.000027 (n = 5 for each group). f PEAK1 regulates metastasis of TNBC cells in vivo. Representative in vivo bioluminescent images of tail vein-injected mice with parental or CRISPR PEAK1 KO cells 1 h and 7 weeks postinjection. g Bioluminescence quantification of lung metastases 7 weeks postinjection of mice bearing parental (n = 8), PEAK1 KO1 (n = 8), or PEAK1 KO2 (n = 8). *P = 0.0439. Data were analyzed from 5 mice (c, e) or 8 mice (g) for each group and expressed as mean ± s.e.m. Two-tailed unpaired t test was used (c, e, g). Source data are provided as a Source data file.